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KK4824 KAPA KAPA 文库定量试剂盒 (Illumina/通用型)

品牌:Roche-KAPA
规格:0
参考价格:6650
剩余数量:00门店价:¥0
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代理产品

品牌:KAPA,~~~~~~~~~~ 规格:~~~~~~~~~~ 价格:6650元~~~~~~~~~~~~~产品详情请参阅KAPA官网,链接:https://www.kapabiosystems.com/

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries.* The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Kits with DNA Standards and Primer Premixes for Illumina® and Ion Torrent™ sequencers are available. Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

NEW! Improve experimental designs with our Technical Guide for Illumina Platforms


*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Accurately quantify PCR-competent sequencing templates

  • qPCR only “counts” those library molecules that can be sequenced
  • More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources
Equimolar pooling of indexed libraries. Eleven indexed Illumina TruSeq libraries were quantified by qPCR using the KAPA Library Quantification Kit, and then combined to achieve equal final concentrations in two separate pools for multiplexed sequencing on different flow-cell lanes. The eleven libraries ranged ~11-fold in concentration from 0.67 pM to 7.65 pM, while representation of each index varied between 90% and 127% of expected assigned reads per lane. Data on file.

Improve pooling for multiplexed sequencing

  • Library concentrations determined by qPCR are more reliable compared to other methods*
  • The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts*
Nine human DNA libraries and two microbial DNA libraries (Rhodococcus sp. ~70% GC and Staphylococcus sp. ~35% GC) were used to compare quantification results obtained with distinct lots (”Lot 1” and “Lot 2”), and distinct sets of reagents from the same lot (”Set 1” and “Set 2”) of KAPA Library Quantification Kits for Illumina platforms. Data on file.

Eliminate drift with carefully quality-controlled DNA Standards

  • Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards*
  • KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Improve throughput with automation

  • Library quantification assay is compatible with 96- and 384-well format
  • Library dilution, reaction setup and data analysis can be automated for HTP pipelines
  • Learn More about KAPA Automated Solutions

*Data on file.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 杭州开泰生物技术有限公司上是一家创办于2006年的创新型高科技企业,自公司成立以来一直专注于为生命科学和生物技术领域的客户提供优质的产品和技术服务。公司是Tiangen,CST,KAPA,科晶和江苏MRC的浙江一级代理。同时公司拥有自主研发的汉泰基因品牌高通量测序产品,并提供各种科研技术服务。如需订购,请在线联系QQ或者致电:0571-88169527.

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